Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
13 |
3.6% |
| Arg[R] |
8 |
2.2% |
| Asn[N] |
36 |
10.0% |
| Asp[D] |
26 |
7.2% |
| Cys[C] |
3 |
0.8% |
| Gln[Q] |
22 |
6.1% |
| Glu[E] |
23 |
6.4% |
| Gly[G] |
6 |
1.7% |
| His[H] |
12 |
3.3% |
| Ile[I] |
19 |
5.3% |
| Leu[L] |
18 |
5.0% |
| Lys[K] |
24 |
6.7% |
| Met[M] |
3 |
0.8% |
| Phe[F] |
26 |
7.2% |
| Pro[P] |
20 |
5.6% |
| Ser[S] |
47 |
13.1% |
| Thr[T] |
25 |
6.9% |
| Trp[W] |
1 |
0.3% |
| Tyr[Y] |
23 |
6.4% |
| Val[V] |
5 |
1.4% |
| Total Length |
360 |
|
| Instability index (II) |
49.33 |
| This classifies the protein as unstable |
|
| Carbon |
1852 |
| Hydrogen |
2731 |
| Nitrogen |
491 |
| Oxygen |
612 |
| Sulfur |
6 |
| Total number of atoms |
5692 |
| Formula = C1852H2731N491O612S6 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
39895 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
39770 M-1 cm-1 |
| assuming all Cys residues are reduced |
39770 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
39895 M-1 cm-1 |
|
|
| Codon Bias |
0.035 |
| Codon Adaptation Index |
0.184 |
| Frequency of Optimal Codons |
0.455 |
| Hydropathicity of Protein |
-1.051 |
| Aromaticity Score |
0.139 |
|
