Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
28 |
2.9% |
| Arg[R] |
49 |
5.1% |
| Asn[N] |
82 |
8.6% |
| Asp[D] |
82 |
8.6% |
| Cys[C] |
12 |
1.3% |
| Gln[Q] |
36 |
3.8% |
| Glu[E] |
69 |
7.2% |
| Gly[G] |
41 |
4.3% |
| His[H] |
19 |
2.0% |
| Ile[I] |
64 |
6.7% |
| Leu[L] |
68 |
7.1% |
| Lys[K] |
91 |
9.5% |
| Met[M] |
16 |
1.7% |
| Phe[F] |
35 |
3.7% |
| Pro[P] |
41 |
4.3% |
| Ser[S] |
95 |
10.0% |
| Thr[T] |
55 |
5.8% |
| Trp[W] |
6 |
0.6% |
| Tyr[Y] |
20 |
2.1% |
| Val[V] |
45 |
4.7% |
| Total Length |
954 |
|
| Instability index (II) |
45.54 |
| This classifies the protein as unstable |
|
| Carbon |
4705 |
| Hydrogen |
7514 |
| Nitrogen |
1354 |
| Oxygen |
1545 |
| Sulfur |
28 |
| Total number of atoms |
15146 |
| Formula = C4705H7514N1354O1545S28 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
63550 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
62800 M-1 cm-1 |
| assuming all Cys residues are reduced |
62800 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
63550 M-1 cm-1 |
|
|
| Codon Bias |
-0.027 |
| Codon Adaptation Index |
0.134 |
| Frequency of Optimal Codons |
0.408 |
| Hydropathicity of Protein |
-0.903 |
| Aromaticity Score |
0.064 |
|
