TOR1/YJR066W Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 156 6.3%
Arg[R] 112 4.5%
Asn[N] 132 5.3%
Asp[D] 124 5.0%
Cys[C] 31 1.3%
Gln[Q] 111 4.5%
Glu[E] 160 6.5%
Gly[G] 93 3.8%
His[H] 61 2.5%
Ile[I] 192 7.8%
Leu[L] 330 13.4%
Lys[K] 164 6.6%
Met[M] 50 2.0%
Phe[F] 89 3.6%
Pro[P] 112 4.5%
Ser[S] 186 7.5%
Thr[T] 128 5.2%
Trp[W] 36 1.5%
Tyr[Y] 76 3.1%
Val[V] 127 5.1%
Total Length 2470

Instability index
Instability index (II) 41.00
This classifies the protein as unstable

Atomic composition
Carbon 12676
Hydrogen 20165
Nitrogen 3371
Oxygen 3672
Sulfur 81
Total number of atoms 39965
Formula = C12676H20165N3371O3672S81

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 313115 M-1 cm-1
assuming NO Cys residues appear as half cystines 311240 M-1 cm-1
assuming all Cys residues are reduced 311240 M-1 cm-1
assuming all pairs of Cys residues form cystines 313115 M-1 cm-1

Aliphatic index
Aliphatic index 103.65

Coding Region Translation Calculations
Codon Bias 0.075
Codon Adaptation Index 0.138
Frequency of Optimal Codons 0.446
Hydropathicity of Protein -0.168
Aromaticity Score 0.081