Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
156 |
6.3% |
| Arg[R] |
112 |
4.5% |
| Asn[N] |
132 |
5.3% |
| Asp[D] |
124 |
5.0% |
| Cys[C] |
31 |
1.3% |
| Gln[Q] |
111 |
4.5% |
| Glu[E] |
160 |
6.5% |
| Gly[G] |
93 |
3.8% |
| His[H] |
61 |
2.5% |
| Ile[I] |
192 |
7.8% |
| Leu[L] |
330 |
13.4% |
| Lys[K] |
164 |
6.6% |
| Met[M] |
50 |
2.0% |
| Phe[F] |
89 |
3.6% |
| Pro[P] |
112 |
4.5% |
| Ser[S] |
186 |
7.5% |
| Thr[T] |
128 |
5.2% |
| Trp[W] |
36 |
1.5% |
| Tyr[Y] |
76 |
3.1% |
| Val[V] |
127 |
5.1% |
| Total Length |
2470 |
|
| Instability index (II) |
41.00 |
| This classifies the protein as unstable |
|
| Carbon |
12676 |
| Hydrogen |
20165 |
| Nitrogen |
3371 |
| Oxygen |
3672 |
| Sulfur |
81 |
| Total number of atoms |
39965 |
| Formula = C12676H20165N3371O3672S81 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
313115 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
311240 M-1 cm-1 |
| assuming all Cys residues are reduced |
311240 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
313115 M-1 cm-1 |
|
|
| Codon Bias |
0.075 |
| Codon Adaptation Index |
0.138 |
| Frequency of Optimal Codons |
0.446 |
| Hydropathicity of Protein |
-0.168 |
| Aromaticity Score |
0.081 |
|