Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
39 |
4.2% |
| Arg[R] |
46 |
4.9% |
| Asn[N] |
71 |
7.6% |
| Asp[D] |
65 |
7.0% |
| Cys[C] |
5 |
0.5% |
| Gln[Q] |
27 |
2.9% |
| Glu[E] |
62 |
6.6% |
| Gly[G] |
41 |
4.4% |
| His[H] |
25 |
2.7% |
| Ile[I] |
53 |
5.7% |
| Leu[L] |
105 |
11.2% |
| Lys[K] |
69 |
7.4% |
| Met[M] |
16 |
1.7% |
| Phe[F] |
55 |
5.9% |
| Pro[P] |
28 |
3.0% |
| Ser[S] |
86 |
9.2% |
| Thr[T] |
41 |
4.4% |
| Trp[W] |
11 |
1.2% |
| Tyr[Y] |
45 |
4.8% |
| Val[V] |
45 |
4.8% |
| Total Length |
935 |
|
| Instability index (II) |
40.52 |
| This classifies the protein as unstable |
|
| Carbon |
4879 |
| Hydrogen |
7511 |
| Nitrogen |
1301 |
| Oxygen |
1460 |
| Sulfur |
21 |
| Total number of atoms |
15172 |
| Formula = C4879H7511N1301O1460S21 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
127800 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
127550 M-1 cm-1 |
| assuming all Cys residues are reduced |
127550 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
127800 M-1 cm-1 |
|
|
| Codon Bias |
0.020 |
| Codon Adaptation Index |
0.126 |
| Frequency of Optimal Codons |
0.421 |
| Hydropathicity of Protein |
-0.510 |
| Aromaticity Score |
0.119 |
|
