Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
65 |
4.8% |
| Arg[R] |
55 |
4.1% |
| Asn[N] |
83 |
6.1% |
| Asp[D] |
85 |
6.3% |
| Cys[C] |
25 |
1.8% |
| Gln[Q] |
60 |
4.4% |
| Glu[E] |
65 |
4.8% |
| Gly[G] |
48 |
3.5% |
| His[H] |
34 |
2.5% |
| Ile[I] |
105 |
7.7% |
| Leu[L] |
147 |
10.8% |
| Lys[K] |
106 |
7.8% |
| Met[M] |
23 |
1.7% |
| Phe[F] |
52 |
3.8% |
| Pro[P] |
65 |
4.8% |
| Ser[S] |
113 |
8.3% |
| Thr[T] |
78 |
5.7% |
| Trp[W] |
24 |
1.8% |
| Tyr[Y] |
44 |
3.2% |
| Val[V] |
80 |
5.9% |
| Total Length |
1357 |
|
| Instability index (II) |
41.17 |
| This classifies the protein as unstable |
|
| Carbon |
6964 |
| Hydrogen |
10993 |
| Nitrogen |
1863 |
| Oxygen |
2036 |
| Sulfur |
48 |
| Total number of atoms |
21904 |
| Formula = C6964H10993N1863O2036S48 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
199060 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
197560 M-1 cm-1 |
| assuming all Cys residues are reduced |
197560 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
199060 M-1 cm-1 |
|
|
| Codon Bias |
0.060 |
| Codon Adaptation Index |
0.144 |
| Frequency of Optimal Codons |
0.449 |
| Hydropathicity of Protein |
-0.299 |
| Aromaticity Score |
0.088 |
|
