Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
43 |
6.6% |
| Arg[R] |
8 |
1.2% |
| Asn[N] |
42 |
6.5% |
| Asp[D] |
29 |
4.5% |
| Cys[C] |
7 |
1.1% |
| Gln[Q] |
20 |
3.1% |
| Glu[E] |
28 |
4.3% |
| Gly[G] |
31 |
4.8% |
| His[H] |
8 |
1.2% |
| Ile[I] |
41 |
6.3% |
| Leu[L] |
49 |
7.5% |
| Lys[K] |
17 |
2.6% |
| Met[M] |
8 |
1.2% |
| Phe[F] |
39 |
6.0% |
| Pro[P] |
19 |
2.9% |
| Ser[S] |
105 |
16.2% |
| Thr[T] |
89 |
13.7% |
| Trp[W] |
7 |
1.1% |
| Tyr[Y] |
27 |
4.2% |
| Val[V] |
33 |
5.1% |
| Total Length |
650 |
|
| Instability index (II) |
31.20 |
| This classifies the protein as stable |
|
| Carbon |
3116 |
| Hydrogen |
4758 |
| Nitrogen |
776 |
| Oxygen |
1048 |
| Sulfur |
15 |
| Total number of atoms |
9713 |
| Formula = C3116H4758N776O1048S15 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
79105 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
78730 M-1 cm-1 |
| assuming all Cys residues are reduced |
78730 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
79105 M-1 cm-1 |
|
|
| Codon Bias |
0.058 |
| Codon Adaptation Index |
0.134 |
| Frequency of Optimal Codons |
0.450 |
| Hydropathicity of Protein |
-0.071 |
| Aromaticity Score |
0.112 |
|
