Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
38 |
6.4% |
| Arg[R] |
27 |
4.6% |
| Asn[N] |
27 |
4.6% |
| Asp[D] |
60 |
10.1% |
| Cys[C] |
1 |
0.2% |
| Gln[Q] |
20 |
3.4% |
| Glu[E] |
80 |
13.5% |
| Gly[G] |
20 |
3.4% |
| His[H] |
6 |
1.0% |
| Ile[I] |
30 |
5.1% |
| Leu[L] |
47 |
7.9% |
| Lys[K] |
57 |
9.6% |
| Met[M] |
9 |
1.5% |
| Phe[F] |
15 |
2.5% |
| Pro[P] |
21 |
3.5% |
| Ser[S] |
64 |
10.8% |
| Thr[T] |
24 |
4.0% |
| Trp[W] |
3 |
0.5% |
| Tyr[Y] |
11 |
1.9% |
| Val[V] |
33 |
5.6% |
| Total Length |
593 |
|
| Instability index (II) |
58.51 |
| This classifies the protein as unstable |
|
| Carbon |
2877 |
| Hydrogen |
4613 |
| Nitrogen |
793 |
| Oxygen |
1020 |
| Sulfur |
10 |
| Total number of atoms |
9313 |
| Formula = C2877H4613N793O1020S10 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
32890 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
32890 M-1 cm-1 |
| assuming all Cys residues are reduced |
32890 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
32890 M-1 cm-1 |
|
|
| Codon Bias |
0.104 |
| Codon Adaptation Index |
0.169 |
| Frequency of Optimal Codons |
0.475 |
| Hydropathicity of Protein |
-0.948 |
| Aromaticity Score |
0.049 |
|
