Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
38 |
6.7% |
| Arg[R] |
20 |
3.5% |
| Asn[N] |
24 |
4.2% |
| Asp[D] |
21 |
3.7% |
| Cys[C] |
11 |
1.9% |
| Gln[Q] |
16 |
2.8% |
| Glu[E] |
19 |
3.4% |
| Gly[G] |
51 |
9.0% |
| His[H] |
4 |
0.7% |
| Ile[I] |
46 |
8.1% |
| Leu[L] |
45 |
7.9% |
| Lys[K] |
25 |
4.4% |
| Met[M] |
17 |
3.0% |
| Phe[F] |
42 |
7.4% |
| Pro[P] |
18 |
3.2% |
| Ser[S] |
47 |
8.3% |
| Thr[T] |
42 |
7.4% |
| Trp[W] |
13 |
2.3% |
| Tyr[Y] |
25 |
4.4% |
| Val[V] |
43 |
7.6% |
| Total Length |
567 |
|
| Instability index (II) |
35.76 |
| This classifies the protein as stable |
|
| Carbon |
2889 |
| Hydrogen |
4407 |
| Nitrogen |
713 |
| Oxygen |
802 |
| Sulfur |
28 |
| Total number of atoms |
8839 |
| Formula = C2889H4407N713O802S28 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
109375 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
108750 M-1 cm-1 |
| assuming all Cys residues are reduced |
108750 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
109375 M-1 cm-1 |
|
|
| Codon Bias |
0.196 |
| Codon Adaptation Index |
0.167 |
| Frequency of Optimal Codons |
0.525 |
| Hydropathicity of Protein |
0.289 |
| Aromaticity Score |
0.141 |
|
