Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
29 |
3.5% |
| Arg[R] |
26 |
3.1% |
| Asn[N] |
79 |
9.4% |
| Asp[D] |
51 |
6.1% |
| Cys[C] |
10 |
1.2% |
| Gln[Q] |
42 |
5.0% |
| Glu[E] |
58 |
6.9% |
| Gly[G] |
21 |
2.5% |
| His[H] |
7 |
0.8% |
| Ile[I] |
81 |
9.6% |
| Leu[L] |
94 |
11.2% |
| Lys[K] |
73 |
8.7% |
| Met[M] |
8 |
1.0% |
| Phe[F] |
55 |
6.5% |
| Pro[P] |
27 |
3.2% |
| Ser[S] |
63 |
7.5% |
| Thr[T] |
40 |
4.8% |
| Trp[W] |
4 |
0.5% |
| Tyr[Y] |
32 |
3.8% |
| Val[V] |
40 |
4.8% |
| Total Length |
840 |
|
| Instability index (II) |
40.95 |
| This classifies the protein as unstable |
|
| Carbon |
4416 |
| Hydrogen |
6932 |
| Nitrogen |
1130 |
| Oxygen |
1315 |
| Sulfur |
18 |
| Total number of atoms |
13811 |
| Formula = C4416H6932N1130O1315S18 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
70305 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
69680 M-1 cm-1 |
| assuming all Cys residues are reduced |
69680 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
70305 M-1 cm-1 |
|
|
| Codon Bias |
0.003 |
| Codon Adaptation Index |
0.152 |
| Frequency of Optimal Codons |
0.434 |
| Hydropathicity of Protein |
-0.319 |
| Aromaticity Score |
0.108 |
|
