Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
60 |
7.6% |
| Arg[R] |
27 |
3.4% |
| Asn[N] |
50 |
6.3% |
| Asp[D] |
47 |
6.0% |
| Cys[C] |
9 |
1.1% |
| Gln[Q] |
25 |
3.2% |
| Glu[E] |
45 |
5.7% |
| Gly[G] |
65 |
8.2% |
| His[H] |
16 |
2.0% |
| Ile[I] |
51 |
6.5% |
| Leu[L] |
67 |
8.5% |
| Lys[K] |
61 |
7.7% |
| Met[M] |
17 |
2.2% |
| Phe[F] |
32 |
4.1% |
| Pro[P] |
41 |
5.2% |
| Ser[S] |
54 |
6.8% |
| Thr[T] |
54 |
6.8% |
| Trp[W] |
9 |
1.1% |
| Tyr[Y] |
21 |
2.7% |
| Val[V] |
38 |
4.8% |
| Total Length |
789 |
|
| Instability index (II) |
27.67 |
| This classifies the protein as stable |
|
| Carbon |
3841 |
| Hydrogen |
6055 |
| Nitrogen |
1047 |
| Oxygen |
1178 |
| Sulfur |
26 |
| Total number of atoms |
12147 |
| Formula = C3841H6055N1047O1178S26 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
81290 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
80790 M-1 cm-1 |
| assuming all Cys residues are reduced |
80790 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
81290 M-1 cm-1 |
|
|
| Codon Bias |
0.230 |
| Codon Adaptation Index |
0.219 |
| Frequency of Optimal Codons |
0.541 |
| Hydropathicity of Protein |
-0.389 |
| Aromaticity Score |
0.079 |
|
