SET2/YJL168C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 39 5.3%
Arg[R] 32 4.4%
Asn[N] 43 5.9%
Asp[D] 46 6.3%
Cys[C] 17 2.3%
Gln[Q] 37 5.0%
Glu[E] 65 8.9%
Gly[G] 28 3.8%
His[H] 14 1.9%
Ile[I] 50 6.8%
Leu[L] 63 8.6%
Lys[K] 91 12.4%
Met[M] 12 1.6%
Phe[F] 32 4.4%
Pro[P] 29 4.0%
Ser[S] 53 7.2%
Thr[T] 29 4.0%
Trp[W] 5 0.7%
Tyr[Y] 20 2.7%
Val[V] 28 3.8%
Total Length 733

Instability index
Instability index (II) 54.69
This classifies the protein as unstable

Atomic composition
Carbon 3733
Hydrogen 5971
Nitrogen 1033
Oxygen 1138
Sulfur 29
Total number of atoms 11904
Formula = C3733H5971N1033O1138S29

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 58300 M-1 cm-1
assuming NO Cys residues appear as half cystines 57300 M-1 cm-1
assuming all Cys residues are reduced 57300 M-1 cm-1
assuming all pairs of Cys residues form cystines 58300 M-1 cm-1

Aliphatic index
Aliphatic index 76.52

Coding Region Translation Calculations
Codon Bias 0.000
Codon Adaptation Index 0.000
Frequency of Optimal Codons 0.000
Hydropathicity of Protein 0.000
Aromaticity Score 0.000