QCR8/YJL166W Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 5 5.3%
Arg[R] 4 4.3%
Asn[N] 6 6.4%
Asp[D] 0 0.0%
Cys[C] 0 0.0%
Gln[Q] 4 4.3%
Glu[E] 5 5.3%
Gly[G] 11 11.7%
His[H] 2 2.1%
Ile[I] 4 4.3%
Leu[L] 5 5.3%
Lys[K] 7 7.4%
Met[M] 3 3.2%
Phe[F] 6 6.4%
Pro[P] 6 6.4%
Ser[S] 6 6.4%
Thr[T] 2 2.1%
Trp[W] 5 5.3%
Tyr[Y] 8 8.5%
Val[V] 5 5.3%
Total Length 94

Instability index
Instability index (II) 39.33
This classifies the protein as stable

Atomic composition
Carbon 515
Hydrogen 742
Nitrogen 132
Oxygen 131
Sulfur 3
Total number of atoms 1523
Formula = C515H742N132O131S3

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 39420 M-1 cm-1

Aliphatic index
Aliphatic index 58.09

Coding Region Translation Calculations
Codon Bias 0.286
Codon Adaptation Index 0.211
Frequency of Optimal Codons 0.570
Hydropathicity of Protein -0.530
Aromaticity Score 0.202