TPK1/YJL164C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 14 3.5%
Arg[R] 20 5.0%
Asn[N] 17 4.3%
Asp[D] 24 6.0%
Cys[C] 2 0.5%
Gln[Q] 23 5.8%
Glu[E] 36 9.1%
Gly[G] 27 6.8%
His[H] 8 2.0%
Ile[I] 24 6.0%
Leu[L] 32 8.1%
Lys[K] 28 7.1%
Met[M] 8 2.0%
Phe[F] 20 5.0%
Pro[P] 19 4.8%
Ser[S] 23 5.8%
Thr[T] 25 6.3%
Trp[W] 5 1.3%
Tyr[Y] 22 5.5%
Val[V] 20 5.0%
Total Length 397

Instability index
Instability index (II) 42.49
This classifies the protein as unstable

Atomic composition
Carbon 2070
Hydrogen 3172
Nitrogen 546
Oxygen 628
Sulfur 10
Total number of atoms 6426
Formula = C2070H3172N546O628S10

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 60405 M-1 cm-1
assuming NO Cys residues appear as half cystines 60280 M-1 cm-1
assuming all Cys residues are reduced 60280 M-1 cm-1
assuming all pairs of Cys residues form cystines 60405 M-1 cm-1

Aliphatic index
Aliphatic index 73.15

Coding Region Translation Calculations
Codon Bias 0.000
Codon Adaptation Index 0.000
Frequency of Optimal Codons 0.000
Hydropathicity of Protein 0.000
Aromaticity Score 0.000