Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
35 |
3.7% |
| Arg[R] |
41 |
4.3% |
| Asn[N] |
60 |
6.4% |
| Asp[D] |
54 |
5.7% |
| Cys[C] |
18 |
1.9% |
| Gln[Q] |
55 |
5.8% |
| Glu[E] |
61 |
6.5% |
| Gly[G] |
25 |
2.6% |
| His[H] |
25 |
2.6% |
| Ile[I] |
78 |
8.3% |
| Leu[L] |
126 |
13.3% |
| Lys[K] |
57 |
6.0% |
| Met[M] |
21 |
2.2% |
| Phe[F] |
35 |
3.7% |
| Pro[P] |
28 |
3.0% |
| Ser[S] |
79 |
8.4% |
| Thr[T] |
52 |
5.5% |
| Trp[W] |
8 |
0.8% |
| Tyr[Y] |
37 |
3.9% |
| Val[V] |
49 |
5.2% |
| Total Length |
944 |
|
| Instability index (II) |
43.71 |
| This classifies the protein as unstable |
|
| Carbon |
4878 |
| Hydrogen |
7747 |
| Nitrogen |
1297 |
| Oxygen |
1458 |
| Sulfur |
39 |
| Total number of atoms |
15419 |
| Formula = C4878H7747N1297O1458S39 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
100255 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
99130 M-1 cm-1 |
| assuming all Cys residues are reduced |
99130 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
100255 M-1 cm-1 |
|
|
| Codon Bias |
0.066 |
| Codon Adaptation Index |
0.147 |
| Frequency of Optimal Codons |
0.454 |
| Hydropathicity of Protein |
-0.233 |
| Aromaticity Score |
0.085 |
|
