Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
31 |
3.8% |
| Arg[R] |
34 |
4.2% |
| Asn[N] |
64 |
7.9% |
| Asp[D] |
32 |
4.0% |
| Cys[C] |
13 |
1.6% |
| Gln[Q] |
68 |
8.4% |
| Glu[E] |
39 |
4.8% |
| Gly[G] |
40 |
5.0% |
| His[H] |
19 |
2.4% |
| Ile[I] |
43 |
5.3% |
| Leu[L] |
69 |
8.6% |
| Lys[K] |
45 |
5.6% |
| Met[M] |
14 |
1.7% |
| Phe[F] |
30 |
3.7% |
| Pro[P] |
54 |
6.7% |
| Ser[S] |
94 |
11.6% |
| Thr[T] |
40 |
5.0% |
| Trp[W] |
7 |
0.9% |
| Tyr[Y] |
35 |
4.3% |
| Val[V] |
36 |
4.5% |
| Total Length |
807 |
|
| Instability index (II) |
54.84 |
| This classifies the protein as unstable |
|
| Carbon |
4015 |
| Hydrogen |
6249 |
| Nitrogen |
1131 |
| Oxygen |
1251 |
| Sulfur |
27 |
| Total number of atoms |
12673 |
| Formula = C4015H6249N1131O1251S27 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
91400 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
90650 M-1 cm-1 |
| assuming all Cys residues are reduced |
90650 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
91400 M-1 cm-1 |
|
|
| Codon Bias |
0.062 |
| Codon Adaptation Index |
0.125 |
| Frequency of Optimal Codons |
0.443 |
| Hydropathicity of Protein |
-0.683 |
| Aromaticity Score |
0.089 |
|
