Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
39 |
5.2% |
| Arg[R] |
24 |
3.2% |
| Asn[N] |
38 |
5.1% |
| Asp[D] |
46 |
6.1% |
| Cys[C] |
7 |
0.9% |
| Gln[Q] |
25 |
3.3% |
| Glu[E] |
35 |
4.7% |
| Gly[G] |
47 |
6.3% |
| His[H] |
19 |
2.5% |
| Ile[I] |
57 |
7.6% |
| Leu[L] |
76 |
10.1% |
| Lys[K] |
44 |
5.9% |
| Met[M] |
18 |
2.4% |
| Phe[F] |
42 |
5.6% |
| Pro[P] |
31 |
4.1% |
| Ser[S] |
70 |
9.3% |
| Thr[T] |
45 |
6.0% |
| Trp[W] |
13 |
1.7% |
| Tyr[Y] |
30 |
4.0% |
| Val[V] |
44 |
5.9% |
| Total Length |
750 |
|
| Instability index (II) |
31.21 |
| This classifies the protein as stable |
|
| Carbon |
3834 |
| Hydrogen |
5906 |
| Nitrogen |
980 |
| Oxygen |
1121 |
| Sulfur |
25 |
| Total number of atoms |
11866 |
| Formula = C3834H5906N980O1121S25 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
116575 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
116200 M-1 cm-1 |
| assuming all Cys residues are reduced |
116200 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
116575 M-1 cm-1 |
|
|
| Codon Bias |
0.015 |
| Codon Adaptation Index |
0.119 |
| Frequency of Optimal Codons |
0.419 |
| Hydropathicity of Protein |
-0.109 |
| Aromaticity Score |
0.113 |
|
