Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
52 |
4.2% |
| Arg[R] |
79 |
6.4% |
| Asn[N] |
83 |
6.7% |
| Asp[D] |
92 |
7.4% |
| Cys[C] |
9 |
0.7% |
| Gln[Q] |
41 |
3.3% |
| Glu[E] |
63 |
5.1% |
| Gly[G] |
70 |
5.7% |
| His[H] |
39 |
3.2% |
| Ile[I] |
75 |
6.1% |
| Leu[L] |
99 |
8.0% |
| Lys[K] |
85 |
6.9% |
| Met[M] |
28 |
2.3% |
| Phe[F] |
55 |
4.5% |
| Pro[P] |
50 |
4.0% |
| Ser[S] |
124 |
10.0% |
| Thr[T] |
77 |
6.2% |
| Trp[W] |
16 |
1.3% |
| Tyr[Y] |
44 |
3.6% |
| Val[V] |
54 |
4.4% |
| Total Length |
1235 |
|
| Instability index (II) |
42.54 |
| This classifies the protein as unstable |
|
| Carbon |
6212 |
| Hydrogen |
9697 |
| Nitrogen |
1775 |
| Oxygen |
1915 |
| Sulfur |
37 |
| Total number of atoms |
19636 |
| Formula = C6212H9697N1775O1915S37 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
154060 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
153560 M-1 cm-1 |
| assuming all Cys residues are reduced |
153560 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
154060 M-1 cm-1 |
|
|
| Codon Bias |
0.024 |
| Codon Adaptation Index |
0.140 |
| Frequency of Optimal Codons |
0.421 |
| Hydropathicity of Protein |
-0.694 |
| Aromaticity Score |
0.093 |
|
