YJL114W Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 11 2.7%
Arg[R] 20 4.8%
Asn[N] 33 8.0%
Asp[D] 29 7.0%
Cys[C] 11 2.7%
Gln[Q] 23 5.6%
Glu[E] 35 8.5%
Gly[G] 12 2.9%
His[H] 6 1.4%
Ile[I] 35 8.5%
Leu[L] 39 9.4%
Lys[K] 39 9.4%
Met[M] 9 2.2%
Phe[F] 13 3.1%
Pro[P] 12 2.9%
Ser[S] 22 5.3%
Thr[T] 27 6.5%
Trp[W] 1 0.2%
Tyr[Y] 12 2.9%
Val[V] 25 6.0%
Total Length 414

Instability index
Instability index (II) 26.39
This classifies the protein as stable

Atomic composition
Carbon 1162
Hydrogen 1888
Nitrogen 320
Oxygen 366
Sulfur 5
Total number of atoms 3741
Formula = C1162H1888N320O366S5

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 24005 M-1 cm-1
assuming NO Cys residues appear as half cystines 23380 M-1 cm-1
assuming all Cys residues are reduced 8940 M-1 cm-1
assuming all pairs of Cys residues form cystines 9065 M-1 cm-1

Aliphatic index
Aliphatic index 97.43

Coding Region Translation Calculations
Codon Bias 0.000
Codon Adaptation Index 0.000
Frequency of Optimal Codons 0.000
Hydropathicity of Protein 0.000
Aromaticity Score 0.000