YJL113W Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 53 2.9%
Arg[R] 81 4.5%
Asn[N] 157 8.7%
Asp[D] 124 6.9%
Cys[C] 27 1.5%
Gln[Q] 57 3.2%
Glu[E] 131 7.3%
Gly[G] 69 3.8%
His[H] 48 2.7%
Ile[I] 164 9.1%
Leu[L] 140 7.8%
Lys[K] 183 10.1%
Met[M] 36 2.0%
Phe[F] 50 2.8%
Pro[P] 57 3.2%
Ser[S] 136 7.5%
Thr[T] 119 6.6%
Trp[W] 9 0.5%
Tyr[Y] 70 3.9%
Val[V] 92 5.1%
Total Length 1803

Instability index
Instability index (II) 41.73
This classifies the protein as unstable

Atomic composition
Carbon 7831
Hydrogen 12517
Nitrogen 2193
Oxygen 2441
Sulfur 54
Total number of atoms 25036
Formula = C7831H12517N2193O2441S54

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 155425 M-1 cm-1
assuming NO Cys residues appear as half cystines 153800 M-1 cm-1
assuming all Cys residues are reduced 144860 M-1 cm-1
assuming all pairs of Cys residues form cystines 146235 M-1 cm-1

Aliphatic index
Aliphatic index 81.20

Coding Region Translation Calculations
Codon Bias 0.000
Codon Adaptation Index 0.000
Frequency of Optimal Codons 0.000
Hydropathicity of Protein 0.000
Aromaticity Score 0.000