Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
39 |
5.5% |
| Arg[R] |
35 |
4.9% |
| Asn[N] |
53 |
7.4% |
| Asp[D] |
51 |
7.1% |
| Cys[C] |
8 |
1.1% |
| Gln[Q] |
29 |
4.1% |
| Glu[E] |
48 |
6.7% |
| Gly[G] |
39 |
5.5% |
| His[H] |
12 |
1.7% |
| Ile[I] |
34 |
4.8% |
| Leu[L] |
76 |
10.6% |
| Lys[K] |
47 |
6.6% |
| Met[M] |
7 |
1.0% |
| Phe[F] |
27 |
3.8% |
| Pro[P] |
18 |
2.5% |
| Ser[S] |
67 |
9.4% |
| Thr[T] |
61 |
8.5% |
| Trp[W] |
7 |
1.0% |
| Tyr[Y] |
20 |
2.8% |
| Val[V] |
36 |
5.0% |
| Total Length |
714 |
|
| Instability index (II) |
42.01 |
| This classifies the protein as unstable |
|
| Carbon |
3494 |
| Hydrogen |
5541 |
| Nitrogen |
979 |
| Oxygen |
1143 |
| Sulfur |
15 |
| Total number of atoms |
11172 |
| Formula = C3494H5541N979O1143S15 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
68800 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
68300 M-1 cm-1 |
| assuming all Cys residues are reduced |
68300 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
68800 M-1 cm-1 |
|
|
| Codon Bias |
0.008 |
| Codon Adaptation Index |
0.121 |
| Frequency of Optimal Codons |
0.410 |
| Hydropathicity of Protein |
-0.579 |
| Aromaticity Score |
0.076 |
|
