Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
95 |
5.4% |
| Arg[R] |
69 |
3.9% |
| Asn[N] |
103 |
5.8% |
| Asp[D] |
83 |
4.7% |
| Cys[C] |
16 |
0.9% |
| Gln[Q] |
48 |
2.7% |
| Glu[E] |
126 |
7.1% |
| Gly[G] |
41 |
2.3% |
| His[H] |
23 |
1.3% |
| Ile[I] |
141 |
8.0% |
| Leu[L] |
257 |
14.5% |
| Lys[K] |
127 |
7.2% |
| Met[M] |
24 |
1.4% |
| Phe[F] |
95 |
5.4% |
| Pro[P] |
45 |
2.5% |
| Ser[S] |
187 |
10.6% |
| Thr[T] |
116 |
6.6% |
| Trp[W] |
10 |
0.6% |
| Tyr[Y] |
44 |
2.5% |
| Val[V] |
119 |
6.7% |
| Total Length |
1769 |
|
| Instability index (II) |
40.44 |
| This classifies the protein as unstable |
|
| Carbon |
9057 |
| Hydrogen |
14570 |
| Nitrogen |
2310 |
| Oxygen |
2686 |
| Sulfur |
40 |
| Total number of atoms |
28663 |
| Formula = C9057H14570N2310O2686S40 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
121560 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
120560 M-1 cm-1 |
| assuming all Cys residues are reduced |
120560 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
121560 M-1 cm-1 |
|
|
| Codon Bias |
0.148 |
| Codon Adaptation Index |
0.170 |
| Frequency of Optimal Codons |
0.495 |
| Hydropathicity of Protein |
0.061 |
| Aromaticity Score |
0.084 |
|
