Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
40 |
3.8% |
| Arg[R] |
40 |
3.8% |
| Asn[N] |
79 |
7.5% |
| Asp[D] |
107 |
10.1% |
| Cys[C] |
10 |
0.9% |
| Gln[Q] |
31 |
2.9% |
| Glu[E] |
127 |
12.0% |
| Gly[G] |
46 |
4.3% |
| His[H] |
19 |
1.8% |
| Ile[I] |
69 |
6.5% |
| Leu[L] |
95 |
9.0% |
| Lys[K] |
62 |
5.9% |
| Met[M] |
28 |
2.6% |
| Phe[F] |
42 |
4.0% |
| Pro[P] |
38 |
3.6% |
| Ser[S] |
86 |
8.1% |
| Thr[T] |
51 |
4.8% |
| Trp[W] |
7 |
0.7% |
| Tyr[Y] |
36 |
3.4% |
| Val[V] |
45 |
4.3% |
| Total Length |
1058 |
|
| Instability index (II) |
47.49 |
| This classifies the protein as unstable |
|
| Carbon |
5282 |
| Hydrogen |
8179 |
| Nitrogen |
1395 |
| Oxygen |
1810 |
| Sulfur |
38 |
| Total number of atoms |
16704 |
| Formula = C5282H8179N1395O1810S38 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
92765 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
92140 M-1 cm-1 |
| assuming all Cys residues are reduced |
92140 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
92765 M-1 cm-1 |
|
|
| Codon Bias |
0.018 |
| Codon Adaptation Index |
0.146 |
| Frequency of Optimal Codons |
0.433 |
| Hydropathicity of Protein |
-0.752 |
| Aromaticity Score |
0.080 |
|
