MRPL49/YJL096W Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 8 5.0%
Arg[R] 14 8.7%
Asn[N] 8 5.0%
Asp[D] 5 3.1%
Cys[C] 0 0.0%
Gln[Q] 3 1.9%
Glu[E] 5 3.1%
Gly[G] 6 3.7%
His[H] 4 2.5%
Ile[I] 11 6.8%
Leu[L] 17 10.6%
Lys[K] 15 9.3%
Met[M] 3 1.9%
Phe[F] 6 3.7%
Pro[P] 8 5.0%
Ser[S] 12 7.5%
Thr[T] 20 12.4%
Trp[W] 1 0.6%
Tyr[Y] 5 3.1%
Val[V] 10 6.2%
Total Length 161

Instability index
Instability index (II) 37.79
This classifies the protein as stable

Atomic composition
Carbon 825
Hydrogen 1358
Nitrogen 238
Oxygen 230
Sulfur 3
Total number of atoms 2654
Formula = C825H1358N238O230S3

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 12950 M-1 cm-1

Aliphatic index
Aliphatic index 90.81

Coding Region Translation Calculations
Codon Bias 0.143
Codon Adaptation Index 0.161
Frequency of Optimal Codons 0.484
Hydropathicity of Protein -0.379
Aromaticity Score 0.075