Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
64 |
5.5% |
| Arg[R] |
69 |
5.9% |
| Asn[N] |
69 |
5.9% |
| Asp[D] |
80 |
6.8% |
| Cys[C] |
8 |
0.7% |
| Gln[Q] |
43 |
3.7% |
| Glu[E] |
83 |
7.1% |
| Gly[G] |
42 |
3.6% |
| His[H] |
25 |
2.1% |
| Ile[I] |
88 |
7.5% |
| Leu[L] |
123 |
10.5% |
| Lys[K] |
108 |
9.2% |
| Met[M] |
21 |
1.8% |
| Phe[F] |
49 |
4.2% |
| Pro[P] |
49 |
4.2% |
| Ser[S] |
106 |
9.0% |
| Thr[T] |
62 |
5.3% |
| Trp[W] |
6 |
0.5% |
| Tyr[Y] |
33 |
2.8% |
| Val[V] |
46 |
3.9% |
| Total Length |
1174 |
|
| Instability index (II) |
47.89 |
| This classifies the protein as unstable |
|
| Carbon |
5954 |
| Hydrogen |
9555 |
| Nitrogen |
1657 |
| Oxygen |
1814 |
| Sulfur |
29 |
| Total number of atoms |
19009 |
| Formula = C5954H9555N1657O1814S29 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
82670 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
82170 M-1 cm-1 |
| assuming all Cys residues are reduced |
82170 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
82670 M-1 cm-1 |
|
|
| Codon Bias |
0.017 |
| Codon Adaptation Index |
0.128 |
| Frequency of Optimal Codons |
0.418 |
| Hydropathicity of Protein |
-0.577 |
| Aromaticity Score |
0.075 |
|
