ALY2/YJL084C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 46 4.4%
Arg[R] 48 4.6%
Asn[N] 94 9.0%
Asp[D] 59 5.6%
Cys[C] 4 0.4%
Gln[Q] 33 3.2%
Glu[E] 65 6.2%
Gly[G] 29 2.8%
His[H] 35 3.3%
Ile[I] 61 5.8%
Leu[L] 94 9.0%
Lys[K] 66 6.3%
Met[M] 12 1.1%
Phe[F] 42 4.0%
Pro[P] 76 7.3%
Ser[S] 151 14.4%
Thr[T] 61 5.8%
Trp[W] 5 0.5%
Tyr[Y] 25 2.4%
Val[V] 40 3.8%
Total Length 1046

Instability index
Instability index (II) 56.18
This classifies the protein as unstable

Atomic composition
Carbon 5129
Hydrogen 8070
Nitrogen 1458
Oxygen 1659
Sulfur 16
Total number of atoms 16332
Formula = C5129H8070N1458O1659S16

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 65000 M-1 cm-1
assuming NO Cys residues appear as half cystines 64750 M-1 cm-1
assuming all Cys residues are reduced 64750 M-1 cm-1
assuming all pairs of Cys residues form cystines 65000 M-1 cm-1

Aliphatic index
Aliphatic index 73.28

Coding Region Translation Calculations
Codon Bias 0.033
Codon Adaptation Index 0.133
Frequency of Optimal Codons 0.423
Hydropathicity of Protein -0.731
Aromaticity Score 0.069