PRY1/YJL079C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 45 15.1%
Arg[R] 2 0.7%
Asn[N] 12 4.0%
Asp[D] 16 5.4%
Cys[C] 4 1.3%
Gln[Q] 8 2.7%
Glu[E] 11 3.7%
Gly[G] 18 6.0%
His[H] 6 2.0%
Ile[I] 4 1.3%
Leu[L] 21 7.0%
Lys[K] 9 3.0%
Met[M] 1 0.3%
Phe[F] 4 1.3%
Pro[P] 14 4.7%
Ser[S] 43 14.4%
Thr[T] 42 14.0%
Trp[W] 5 1.7%
Tyr[Y] 13 4.3%
Val[V] 21 7.0%
Total Length 299

Instability index
Instability index (II) 30.42
This classifies the protein as stable

Atomic composition
Carbon 1327
Hydrogen 2051
Nitrogen 351
Oxygen 472
Sulfur 5
Total number of atoms 4206
Formula = C1327H2051N351O472S5

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 47120 M-1 cm-1
assuming NO Cys residues appear as half cystines 46870 M-1 cm-1
assuming all Cys residues are reduced 46870 M-1 cm-1
assuming all pairs of Cys residues form cystines 47120 M-1 cm-1

Aliphatic index
Aliphatic index 68.03

Coding Region Translation Calculations
Codon Bias 0.405
Codon Adaptation Index 0.297
Frequency of Optimal Codons 0.635
Hydropathicity of Protein -0.176
Aromaticity Score 0.074