Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
44 |
5.0% |
| Arg[R] |
23 |
2.6% |
| Asn[N] |
83 |
9.4% |
| Asp[D] |
65 |
7.4% |
| Cys[C] |
41 |
4.7% |
| Gln[Q] |
35 |
4.0% |
| Glu[E] |
40 |
4.5% |
| Gly[G] |
31 |
3.5% |
| His[H] |
62 |
7.0% |
| Ile[I] |
38 |
4.3% |
| Leu[L] |
60 |
6.8% |
| Lys[K] |
69 |
7.8% |
| Met[M] |
10 |
1.1% |
| Phe[F] |
30 |
3.4% |
| Pro[P] |
52 |
5.9% |
| Ser[S] |
95 |
10.8% |
| Thr[T] |
54 |
6.1% |
| Trp[W] |
7 |
0.8% |
| Tyr[Y] |
19 |
2.2% |
| Val[V] |
22 |
2.5% |
| Total Length |
880 |
|
| Instability index (II) |
43.58 |
| This classifies the protein as unstable |
|
| Carbon |
4235 |
| Hydrogen |
6535 |
| Nitrogen |
1267 |
| Oxygen |
1377 |
| Sulfur |
51 |
| Total number of atoms |
13465 |
| Formula = C4235H6535N1267O1377S51 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
69310 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
66810 M-1 cm-1 |
| assuming all Cys residues are reduced |
66810 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
69310 M-1 cm-1 |
|
|
| Codon Bias |
0.013 |
| Codon Adaptation Index |
0.161 |
| Frequency of Optimal Codons |
0.428 |
| Hydropathicity of Protein |
-0.927 |
| Aromaticity Score |
0.064 |
|
