Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
10 |
2.5% |
| Arg[R] |
19 |
4.8% |
| Asn[N] |
35 |
8.8% |
| Asp[D] |
19 |
4.8% |
| Cys[C] |
4 |
1.0% |
| Gln[Q] |
23 |
5.8% |
| Glu[E] |
20 |
5.1% |
| Gly[G] |
24 |
6.1% |
| His[H] |
7 |
1.8% |
| Ile[I] |
13 |
3.3% |
| Leu[L] |
43 |
10.9% |
| Lys[K] |
24 |
6.1% |
| Met[M] |
8 |
2.0% |
| Phe[F] |
13 |
3.3% |
| Pro[P] |
17 |
4.3% |
| Ser[S] |
47 |
11.9% |
| Thr[T] |
24 |
6.1% |
| Trp[W] |
7 |
1.8% |
| Tyr[Y] |
18 |
4.5% |
| Val[V] |
21 |
5.3% |
| Total Length |
396 |
|
| Instability index (II) |
44.76 |
| This classifies the protein as unstable |
|
| Carbon |
1980 |
| Hydrogen |
3086 |
| Nitrogen |
556 |
| Oxygen |
622 |
| Sulfur |
12 |
| Total number of atoms |
6256 |
| Formula = C1980H3086N556O622S12 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
65570 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
65320 M-1 cm-1 |
| assuming all Cys residues are reduced |
65320 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
65570 M-1 cm-1 |
|
|
| Codon Bias |
0.120 |
| Codon Adaptation Index |
0.136 |
| Frequency of Optimal Codons |
0.470 |
| Hydropathicity of Protein |
-0.687 |
| Aromaticity Score |
0.096 |
|
