Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
57 |
8.4% |
| Arg[R] |
20 |
2.9% |
| Asn[N] |
34 |
5.0% |
| Asp[D] |
59 |
8.7% |
| Cys[C] |
1 |
0.1% |
| Gln[Q] |
21 |
3.1% |
| Glu[E] |
55 |
8.1% |
| Gly[G] |
53 |
7.8% |
| His[H] |
8 |
1.2% |
| Ile[I] |
43 |
6.3% |
| Leu[L] |
62 |
9.1% |
| Lys[K] |
60 |
8.8% |
| Met[M] |
9 |
1.3% |
| Phe[F] |
25 |
3.7% |
| Pro[P] |
20 |
2.9% |
| Ser[S] |
39 |
5.7% |
| Thr[T] |
40 |
5.9% |
| Trp[W] |
1 |
0.1% |
| Tyr[Y] |
18 |
2.6% |
| Val[V] |
57 |
8.4% |
| Total Length |
682 |
|
| Instability index (II) |
26.90 |
| This classifies the protein as stable |
|
| Carbon |
3295 |
| Hydrogen |
5280 |
| Nitrogen |
874 |
| Oxygen |
1063 |
| Sulfur |
10 |
| Total number of atoms |
10522 |
| Formula = C3295H5280N874O1063S10 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
32320 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
32320 M-1 cm-1 |
| assuming all Cys residues are reduced |
32320 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
32320 M-1 cm-1 |
|
|
| Codon Bias |
0.481 |
| Codon Adaptation Index |
0.440 |
| Frequency of Optimal Codons |
0.695 |
| Hydropathicity of Protein |
-0.318 |
| Aromaticity Score |
0.065 |
|
