Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
22 |
3.2% |
| Arg[R] |
27 |
4.0% |
| Asn[N] |
49 |
7.2% |
| Asp[D] |
49 |
7.2% |
| Cys[C] |
2 |
0.3% |
| Gln[Q] |
51 |
7.5% |
| Glu[E] |
52 |
7.6% |
| Gly[G] |
28 |
4.1% |
| His[H] |
16 |
2.3% |
| Ile[I] |
42 |
6.2% |
| Leu[L] |
59 |
8.7% |
| Lys[K] |
47 |
6.9% |
| Met[M] |
12 |
1.8% |
| Phe[F] |
28 |
4.1% |
| Pro[P] |
25 |
3.7% |
| Ser[S] |
66 |
9.7% |
| Thr[T] |
36 |
5.3% |
| Trp[W] |
9 |
1.3% |
| Tyr[Y] |
33 |
4.8% |
| Val[V] |
29 |
4.3% |
| Total Length |
682 |
|
| Instability index (II) |
52.56 |
| This classifies the protein as unstable |
|
| Carbon |
3501 |
| Hydrogen |
5393 |
| Nitrogen |
951 |
| Oxygen |
1120 |
| Sulfur |
14 |
| Total number of atoms |
10979 |
| Formula = C3501H5393N951O1120S14 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
98795 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
98670 M-1 cm-1 |
| assuming all Cys residues are reduced |
98670 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
98795 M-1 cm-1 |
|
|
| Codon Bias |
0.015 |
| Codon Adaptation Index |
0.126 |
| Frequency of Optimal Codons |
0.431 |
| Hydropathicity of Protein |
-0.820 |
| Aromaticity Score |
0.103 |
|
