YJL015C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 2 2.1%
Arg[R] 9 9.6%
Asn[N] 4 4.3%
Asp[D] 2 2.1%
Cys[C] 6 6.4%
Gln[Q] 2 2.1%
Glu[E] 2 2.1%
Gly[G] 5 5.3%
His[H] 5 5.3%
Ile[I] 11 11.7%
Leu[L] 7 7.4%
Lys[K] 5 5.3%
Met[M] 2 2.1%
Phe[F] 6 6.4%
Pro[P] 0 0.0%
Ser[S] 5 5.3%
Thr[T] 8 8.5%
Trp[W] 2 2.1%
Tyr[Y] 6 6.4%
Val[V] 5 5.3%
Total Length 94

Instability index
Instability index (II) 46.12
This classifies the protein as unstable

Atomic composition
Carbon 512
Hydrogen 794
Nitrogen 144
Oxygen 128
Sulfur 8
Total number of atoms 1586
Formula = C512H794N144O128S8

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 28795 M-1 cm-1
assuming NO Cys residues appear as half cystines 28420 M-1 cm-1
assuming all Cys residues are reduced 19940 M-1 cm-1
assuming all pairs of Cys residues form cystines 20315 M-1 cm-1

Aliphatic index
Aliphatic index 92.23

Coding Region Translation Calculations
Codon Bias 0.000
Codon Adaptation Index 0.000
Frequency of Optimal Codons 0.000
Hydropathicity of Protein 0.000
Aromaticity Score 0.000