Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
35 |
4.9% |
| Arg[R] |
42 |
5.8% |
| Asn[N] |
33 |
4.6% |
| Asp[D] |
52 |
7.2% |
| Cys[C] |
4 |
0.6% |
| Gln[Q] |
24 |
3.3% |
| Glu[E] |
52 |
7.2% |
| Gly[G] |
35 |
4.9% |
| His[H] |
15 |
2.1% |
| Ile[I] |
46 |
6.4% |
| Leu[L] |
63 |
8.7% |
| Lys[K] |
57 |
7.9% |
| Met[M] |
11 |
1.5% |
| Phe[F] |
39 |
5.4% |
| Pro[P] |
32 |
4.4% |
| Ser[S] |
43 |
6.0% |
| Thr[T] |
44 |
6.1% |
| Trp[W] |
9 |
1.2% |
| Tyr[Y] |
29 |
4.0% |
| Val[V] |
56 |
7.8% |
| Total Length |
721 |
|
| Instability index (II) |
34.79 |
| This classifies the protein as stable |
|
| Carbon |
3756 |
| Hydrogen |
5860 |
| Nitrogen |
1000 |
| Oxygen |
1103 |
| Sulfur |
15 |
| Total number of atoms |
11734 |
| Formula = C3756H5860N1000O1103S15 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
92960 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
92710 M-1 cm-1 |
| assuming all Cys residues are reduced |
92710 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
92960 M-1 cm-1 |
|
|
| Codon Bias |
0.223 |
| Codon Adaptation Index |
0.247 |
| Frequency of Optimal Codons |
0.545 |
| Hydropathicity of Protein |
-0.436 |
| Aromaticity Score |
0.107 |
|
