Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
94 |
4.6% |
| Arg[R] |
102 |
5.0% |
| Asn[N] |
155 |
7.7% |
| Asp[D] |
125 |
6.2% |
| Cys[C] |
20 |
1.0% |
| Gln[Q] |
63 |
3.1% |
| Glu[E] |
118 |
5.8% |
| Gly[G] |
82 |
4.0% |
| His[H] |
55 |
2.7% |
| Ile[I] |
112 |
5.5% |
| Leu[L] |
211 |
10.4% |
| Lys[K] |
135 |
6.7% |
| Met[M] |
51 |
2.5% |
| Phe[F] |
74 |
3.7% |
| Pro[P] |
87 |
4.3% |
| Ser[S] |
241 |
11.9% |
| Thr[T] |
133 |
6.6% |
| Trp[W] |
12 |
0.6% |
| Tyr[Y] |
55 |
2.7% |
| Val[V] |
101 |
5.0% |
| Total Length |
2026 |
|
| Instability index (II) |
49.08 |
| This classifies the protein as unstable |
|
| Carbon |
9964 |
| Hydrogen |
15879 |
| Nitrogen |
2807 |
| Oxygen |
3160 |
| Sulfur |
71 |
| Total number of atoms |
31881 |
| Formula = C9964H15879N2807O3160S71 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
149200 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
147950 M-1 cm-1 |
| assuming all Cys residues are reduced |
147950 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
149200 M-1 cm-1 |
|
|
| Codon Bias |
0.021 |
| Codon Adaptation Index |
0.125 |
| Frequency of Optimal Codons |
0.415 |
| Hydropathicity of Protein |
-0.524 |
| Aromaticity Score |
0.070 |
|
