YHR214W-A Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 7 4.3%
Arg[R] 3 1.9%
Asn[N] 5 3.1%
Asp[D] 1 0.6%
Cys[C] 0 0.0%
Gln[Q] 14 8.7%
Glu[E] 0 0.0%
Gly[G] 0 0.0%
His[H] 8 5.0%
Ile[I] 1 0.6%
Leu[L] 41 25.5%
Lys[K] 6 3.7%
Met[M] 3 1.9%
Phe[F] 6 3.7%
Pro[P] 25 15.5%
Ser[S] 9 5.6%
Thr[T] 17 10.6%
Trp[W] 3 1.9%
Tyr[Y] 0 0.0%
Val[V] 12 7.5%
Total Length 161

Instability index
Instability index (II) 58.78
This classifies the protein as unstable

Atomic composition
Carbon 851
Hydrogen 1368
Nitrogen 214
Oxygen 209
Sulfur 3
Total number of atoms 2645
Formula = C851H1368N214O209S3

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 16500 M-1 cm-1

Aliphatic index
Aliphatic index 127.70

Coding Region Translation Calculations
Codon Bias -0.103
Codon Adaptation Index 0.044
Frequency of Optimal Codons 0.277
Hydropathicity of Protein 0.320
Aromaticity Score 0.056