Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
25 |
4.3% |
| Arg[R] |
26 |
4.5% |
| Asn[N] |
29 |
5.0% |
| Asp[D] |
45 |
7.7% |
| Cys[C] |
5 |
0.9% |
| Gln[Q] |
17 |
2.9% |
| Glu[E] |
43 |
7.4% |
| Gly[G] |
37 |
6.3% |
| His[H] |
14 |
2.4% |
| Ile[I] |
35 |
6.0% |
| Leu[L] |
38 |
6.5% |
| Lys[K] |
46 |
7.9% |
| Met[M] |
8 |
1.4% |
| Phe[F] |
41 |
7.0% |
| Pro[P] |
28 |
4.8% |
| Ser[S] |
44 |
7.5% |
| Thr[T] |
33 |
5.7% |
| Trp[W] |
20 |
3.4% |
| Tyr[Y] |
26 |
4.5% |
| Val[V] |
24 |
4.1% |
| Total Length |
584 |
|
| Instability index (II) |
30.15 |
| This classifies the protein as stable |
|
| Carbon |
3101 |
| Hydrogen |
4602 |
| Nitrogen |
802 |
| Oxygen |
910 |
| Sulfur |
13 |
| Total number of atoms |
9428 |
| Formula = C3101H4602N802O910S13 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
148990 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
148740 M-1 cm-1 |
| assuming all Cys residues are reduced |
148740 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
148990 M-1 cm-1 |
|
|
| Codon Bias |
0.190 |
| Codon Adaptation Index |
0.225 |
| Frequency of Optimal Codons |
0.529 |
| Hydropathicity of Protein |
-0.667 |
| Aromaticity Score |
0.149 |
|
