RTT102/YGR275W Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 8 5.1%
Arg[R] 7 4.5%
Asn[N] 13 8.3%
Asp[D] 13 8.3%
Cys[C] 1 0.6%
Gln[Q] 10 6.4%
Glu[E] 11 7.0%
Gly[G] 9 5.7%
His[H] 1 0.6%
Ile[I] 5 3.2%
Leu[L] 11 7.0%
Lys[K] 15 9.6%
Met[M] 4 2.5%
Phe[F] 2 1.3%
Pro[P] 7 4.5%
Ser[S] 15 9.6%
Thr[T] 9 5.7%
Trp[W] 3 1.9%
Tyr[Y] 6 3.8%
Val[V] 7 4.5%
Total Length 157

Instability index
Instability index (II) 39.69
This classifies the protein as stable

Atomic composition
Carbon 764
Hydrogen 1209
Nitrogen 221
Oxygen 259
Sulfur 5
Total number of atoms 2458
Formula = C764H1209N221O259S5

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 25440 M-1 cm-1
assuming NO Cys residues appear as half cystines 25440 M-1 cm-1
assuming all Cys residues are reduced 25440 M-1 cm-1
assuming all pairs of Cys residues form cystines 25440 M-1 cm-1

Aliphatic index
Aliphatic index 57.77

Coding Region Translation Calculations
Codon Bias -0.026
Codon Adaptation Index 0.120
Frequency of Optimal Codons 0.393
Hydropathicity of Protein -1.131
Aromaticity Score 0.070