Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
49 |
4.8% |
| Arg[R] |
51 |
4.9% |
| Asn[N] |
66 |
6.4% |
| Asp[D] |
83 |
8.1% |
| Cys[C] |
4 |
0.4% |
| Gln[Q] |
45 |
4.4% |
| Glu[E] |
100 |
9.7% |
| Gly[G] |
51 |
4.9% |
| His[H] |
10 |
1.0% |
| Ile[I] |
57 |
5.5% |
| Leu[L] |
83 |
8.1% |
| Lys[K] |
97 |
9.4% |
| Met[M] |
25 |
2.4% |
| Phe[F] |
48 |
4.7% |
| Pro[P] |
41 |
4.0% |
| Ser[S] |
86 |
8.3% |
| Thr[T] |
51 |
4.9% |
| Trp[W] |
10 |
1.0% |
| Tyr[Y] |
23 |
2.2% |
| Val[V] |
51 |
4.9% |
| Total Length |
1031 |
|
| Instability index (II) |
45.20 |
| This classifies the protein as unstable |
|
| Carbon |
5166 |
| Hydrogen |
8174 |
| Nitrogen |
1422 |
| Oxygen |
1669 |
| Sulfur |
29 |
| Total number of atoms |
16460 |
| Formula = C5166H8174N1422O1669S29 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
89520 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
89270 M-1 cm-1 |
| assuming all Cys residues are reduced |
89270 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
89520 M-1 cm-1 |
|
|
| Codon Bias |
0.025 |
| Codon Adaptation Index |
0.127 |
| Frequency of Optimal Codons |
0.430 |
| Hydropathicity of Protein |
-0.807 |
| Aromaticity Score |
0.079 |
|
