Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
16 |
3.8% |
| Arg[R] |
28 |
6.6% |
| Asn[N] |
38 |
9.0% |
| Asp[D] |
35 |
8.3% |
| Cys[C] |
4 |
0.9% |
| Gln[Q] |
17 |
4.0% |
| Glu[E] |
34 |
8.0% |
| Gly[G] |
13 |
3.1% |
| His[H] |
7 |
1.7% |
| Ile[I] |
17 |
4.0% |
| Leu[L] |
37 |
8.7% |
| Lys[K] |
47 |
11.1% |
| Met[M] |
4 |
0.9% |
| Phe[F] |
19 |
4.5% |
| Pro[P] |
19 |
4.5% |
| Ser[S] |
27 |
6.4% |
| Thr[T] |
34 |
8.0% |
| Trp[W] |
3 |
0.7% |
| Tyr[Y] |
11 |
2.6% |
| Val[V] |
14 |
3.3% |
| Total Length |
424 |
|
| Instability index (II) |
32.29 |
| This classifies the protein as stable |
|
| Carbon |
2154 |
| Hydrogen |
3429 |
| Nitrogen |
627 |
| Oxygen |
690 |
| Sulfur |
8 |
| Total number of atoms |
6908 |
| Formula = C2154H3429N627O690S8 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
33140 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
32890 M-1 cm-1 |
| assuming all Cys residues are reduced |
32890 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
33140 M-1 cm-1 |
|
|
| Codon Bias |
0.106 |
| Codon Adaptation Index |
0.157 |
| Frequency of Optimal Codons |
0.477 |
| Hydropathicity of Protein |
-1.151 |
| Aromaticity Score |
0.078 |
|
