Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
46 |
6.3% |
| Arg[R] |
44 |
6.0% |
| Asn[N] |
53 |
7.2% |
| Asp[D] |
55 |
7.5% |
| Cys[C] |
2 |
0.3% |
| Gln[Q] |
20 |
2.7% |
| Glu[E] |
76 |
10.3% |
| Gly[G] |
48 |
6.5% |
| His[H] |
7 |
1.0% |
| Ile[I] |
29 |
3.9% |
| Leu[L] |
45 |
6.1% |
| Lys[K] |
75 |
10.2% |
| Met[M] |
18 |
2.4% |
| Phe[F] |
18 |
2.4% |
| Pro[P] |
40 |
5.4% |
| Ser[S] |
55 |
7.5% |
| Thr[T] |
44 |
6.0% |
| Trp[W] |
4 |
0.5% |
| Tyr[Y] |
15 |
2.0% |
| Val[V] |
41 |
5.6% |
| Total Length |
735 |
|
| Instability index (II) |
47.78 |
| This classifies the protein as unstable |
|
| Carbon |
3529 |
| Hydrogen |
5693 |
| Nitrogen |
1033 |
| Oxygen |
1185 |
| Sulfur |
20 |
| Total number of atoms |
11460 |
| Formula = C3529H5693N1033O1185S20 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
44475 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
44350 M-1 cm-1 |
| assuming all Cys residues are reduced |
44350 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
44475 M-1 cm-1 |
|
|
| Codon Bias |
0.061 |
| Codon Adaptation Index |
0.147 |
| Frequency of Optimal Codons |
0.442 |
| Hydropathicity of Protein |
-1.037 |
| Aromaticity Score |
0.050 |
|
