QCR9/YGR183C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 7 10.6%
Arg[R] 2 3.0%
Asn[N] 3 4.5%
Asp[D] 7 10.6%
Cys[C] 0 0.0%
Gln[Q] 1 1.5%
Glu[E] 2 3.0%
Gly[G] 5 7.6%
His[H] 1 1.5%
Ile[I] 3 4.5%
Leu[L] 2 3.0%
Lys[K] 6 9.1%
Met[M] 1 1.5%
Phe[F] 8 12.1%
Pro[P] 0 0.0%
Ser[S] 4 6.1%
Thr[T] 5 7.6%
Trp[W] 2 3.0%
Tyr[Y] 2 3.0%
Val[V] 5 7.6%
Total Length 66

Instability index
Instability index (II) 26.88
This classifies the protein as stable

Atomic composition
Carbon 344
Hydrogen 504
Nitrogen 86
Oxygen 100
Sulfur 1
Total number of atoms 1035
Formula = C344H504N86O100S1

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 13980 M-1 cm-1

Aliphatic index
Aliphatic index 62.12

Coding Region Translation Calculations
Codon Bias 0.028
Codon Adaptation Index 0.185
Frequency of Optimal Codons 0.444
Hydropathicity of Protein -0.230
Aromaticity Score 0.182