Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
52 |
4.6% |
| Arg[R] |
63 |
5.5% |
| Asn[N] |
78 |
6.9% |
| Asp[D] |
68 |
6.0% |
| Cys[C] |
15 |
1.3% |
| Gln[Q] |
29 |
2.5% |
| Glu[E] |
79 |
6.9% |
| Gly[G] |
47 |
4.1% |
| His[H] |
28 |
2.5% |
| Ile[I] |
82 |
7.2% |
| Leu[L] |
87 |
7.6% |
| Lys[K] |
87 |
7.6% |
| Met[M] |
21 |
1.8% |
| Phe[F] |
60 |
5.3% |
| Pro[P] |
50 |
4.4% |
| Ser[S] |
119 |
10.5% |
| Thr[T] |
68 |
6.0% |
| Trp[W] |
13 |
1.1% |
| Tyr[Y] |
36 |
3.2% |
| Val[V] |
56 |
4.9% |
| Total Length |
1138 |
|
| Instability index (II) |
43.88 |
| This classifies the protein as unstable |
|
| Carbon |
5772 |
| Hydrogen |
9034 |
| Nitrogen |
1590 |
| Oxygen |
1763 |
| Sulfur |
36 |
| Total number of atoms |
18195 |
| Formula = C5772H9034N1590O1763S36 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
126015 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
125140 M-1 cm-1 |
| assuming all Cys residues are reduced |
125140 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
126015 M-1 cm-1 |
|
|
| Codon Bias |
0.004 |
| Codon Adaptation Index |
0.130 |
| Frequency of Optimal Codons |
0.418 |
| Hydropathicity of Protein |
-0.552 |
| Aromaticity Score |
0.096 |
|
