Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
59 |
6.2% |
| Arg[R] |
57 |
6.0% |
| Asn[N] |
59 |
6.2% |
| Asp[D] |
50 |
5.3% |
| Cys[C] |
4 |
0.4% |
| Gln[Q] |
42 |
4.4% |
| Glu[E] |
109 |
11.4% |
| Gly[G] |
54 |
5.7% |
| His[H] |
14 |
1.5% |
| Ile[I] |
36 |
3.8% |
| Leu[L] |
55 |
5.8% |
| Lys[K] |
88 |
9.2% |
| Met[M] |
17 |
1.8% |
| Phe[F] |
25 |
2.6% |
| Pro[P] |
56 |
5.9% |
| Ser[S] |
85 |
8.9% |
| Thr[T] |
74 |
7.8% |
| Trp[W] |
7 |
0.7% |
| Tyr[Y] |
23 |
2.4% |
| Val[V] |
38 |
4.0% |
| Total Length |
952 |
|
| Instability index (II) |
57.07 |
| This classifies the protein as unstable |
|
| Carbon |
4603 |
| Hydrogen |
7353 |
| Nitrogen |
1347 |
| Oxygen |
1554 |
| Sulfur |
21 |
| Total number of atoms |
14878 |
| Formula = C4603H7353N1347O1554S21 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
73020 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
72770 M-1 cm-1 |
| assuming all Cys residues are reduced |
72770 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
73020 M-1 cm-1 |
|
|
| Codon Bias |
0.324 |
| Codon Adaptation Index |
0.254 |
| Frequency of Optimal Codons |
0.598 |
| Hydropathicity of Protein |
-1.127 |
| Aromaticity Score |
0.058 |
|
