Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
46 |
6.0% |
| Arg[R] |
30 |
3.9% |
| Asn[N] |
67 |
8.7% |
| Asp[D] |
54 |
7.0% |
| Cys[C] |
6 |
0.8% |
| Gln[Q] |
27 |
3.5% |
| Glu[E] |
39 |
5.1% |
| Gly[G] |
52 |
6.7% |
| His[H] |
17 |
2.2% |
| Ile[I] |
35 |
4.5% |
| Leu[L] |
53 |
6.9% |
| Lys[K] |
22 |
2.9% |
| Met[M] |
15 |
1.9% |
| Phe[F] |
36 |
4.7% |
| Pro[P] |
43 |
5.6% |
| Ser[S] |
93 |
12.1% |
| Thr[T] |
48 |
6.2% |
| Trp[W] |
14 |
1.8% |
| Tyr[Y] |
45 |
5.8% |
| Val[V] |
29 |
3.8% |
| Total Length |
771 |
|
| Instability index (II) |
54.75 |
| This classifies the protein as unstable |
|
| Carbon |
3805 |
| Hydrogen |
5657 |
| Nitrogen |
1025 |
| Oxygen |
1238 |
| Sulfur |
21 |
| Total number of atoms |
11746 |
| Formula = C3805H5657N1025O1238S21 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
144425 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
144050 M-1 cm-1 |
| assuming all Cys residues are reduced |
144050 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
144425 M-1 cm-1 |
|
|
| Codon Bias |
0.076 |
| Codon Adaptation Index |
0.137 |
| Frequency of Optimal Codons |
0.447 |
| Hydropathicity of Protein |
-0.636 |
| Aromaticity Score |
0.123 |
|
