PRE9/YGR135W Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 24 9.3%
Arg[R] 12 4.7%
Asn[N] 8 3.1%
Asp[D] 22 8.5%
Cys[C] 0 0.0%
Gln[Q] 10 3.9%
Glu[E] 17 6.6%
Gly[G] 18 7.0%
His[H] 3 1.2%
Ile[I] 20 7.8%
Leu[L] 22 8.5%
Lys[K] 19 7.4%
Met[M] 5 1.9%
Phe[F] 5 1.9%
Pro[P] 5 1.9%
Ser[S] 17 6.6%
Thr[T] 22 8.5%
Trp[W] 1 0.4%
Tyr[Y] 16 6.2%
Val[V] 12 4.7%
Total Length 258

Instability index
Instability index (II) 30.46
This classifies the protein as stable

Atomic composition
Carbon 1268
Hydrogen 2014
Nitrogen 338
Oxygen 410
Sulfur 5
Total number of atoms 4035
Formula = C1268H2014N338O410S5

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 29340 M-1 cm-1

Aliphatic index
Aliphatic index 86.28

Coding Region Translation Calculations
Codon Bias 0.186
Codon Adaptation Index 0.182
Frequency of Optimal Codons 0.520
Hydropathicity of Protein -0.436
Aromaticity Score 0.085