Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
77 |
5.3% |
| Arg[R] |
74 |
5.1% |
| Asn[N] |
99 |
6.8% |
| Asp[D] |
123 |
8.5% |
| Cys[C] |
7 |
0.5% |
| Gln[Q] |
59 |
4.1% |
| Glu[E] |
154 |
10.6% |
| Gly[G] |
56 |
3.9% |
| His[H] |
33 |
2.3% |
| Ile[I] |
103 |
7.1% |
| Leu[L] |
123 |
8.5% |
| Lys[K] |
116 |
8.0% |
| Met[M] |
20 |
1.4% |
| Phe[F] |
58 |
4.0% |
| Pro[P] |
45 |
3.1% |
| Ser[S] |
99 |
6.8% |
| Thr[T] |
63 |
4.3% |
| Trp[W] |
11 |
0.8% |
| Tyr[Y] |
61 |
4.2% |
| Val[V] |
70 |
4.8% |
| Total Length |
1451 |
|
| Instability index (II) |
47.34 |
| This classifies the protein as unstable |
|
| Carbon |
7427 |
| Hydrogen |
11588 |
| Nitrogen |
2024 |
| Oxygen |
2387 |
| Sulfur |
27 |
| Total number of atoms |
23453 |
| Formula = C7427H11588N2024O2387S27 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
151765 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
151390 M-1 cm-1 |
| assuming all Cys residues are reduced |
151390 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
151765 M-1 cm-1 |
|
|
| Codon Bias |
0.179 |
| Codon Adaptation Index |
0.218 |
| Frequency of Optimal Codons |
0.526 |
| Hydropathicity of Protein |
-0.785 |
| Aromaticity Score |
0.090 |
|
