Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
60 |
6.3% |
| Arg[R] |
50 |
5.3% |
| Asn[N] |
44 |
4.6% |
| Asp[D] |
63 |
6.6% |
| Cys[C] |
10 |
1.1% |
| Gln[Q] |
36 |
3.8% |
| Glu[E] |
79 |
8.3% |
| Gly[G] |
40 |
4.2% |
| His[H] |
16 |
1.7% |
| Ile[I] |
62 |
6.5% |
| Leu[L] |
94 |
9.9% |
| Lys[K] |
64 |
6.7% |
| Met[M] |
24 |
2.5% |
| Phe[F] |
58 |
6.1% |
| Pro[P] |
30 |
3.2% |
| Ser[S] |
73 |
7.7% |
| Thr[T] |
52 |
5.5% |
| Trp[W] |
9 |
0.9% |
| Tyr[Y] |
36 |
3.8% |
| Val[V] |
50 |
5.3% |
| Total Length |
950 |
|
| Instability index (II) |
49.04 |
| This classifies the protein as unstable |
|
| Carbon |
4901 |
| Hydrogen |
7629 |
| Nitrogen |
1285 |
| Oxygen |
1476 |
| Sulfur |
34 |
| Total number of atoms |
15325 |
| Formula = C4901H7629N1285O1476S34 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
103765 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
103140 M-1 cm-1 |
| assuming all Cys residues are reduced |
103140 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
103765 M-1 cm-1 |
|
|
| Codon Bias |
0.053 |
| Codon Adaptation Index |
0.146 |
| Frequency of Optimal Codons |
0.442 |
| Hydropathicity of Protein |
-0.347 |
| Aromaticity Score |
0.108 |
|
