TOM20/YGR082W Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 24 13.1%
Arg[R] 9 4.9%
Asn[N] 6 3.3%
Asp[D] 8 4.4%
Cys[C] 0 0.0%
Gln[Q] 11 6.0%
Glu[E] 16 8.7%
Gly[G] 7 3.8%
His[H] 1 0.5%
Ile[I] 13 7.1%
Leu[L] 14 7.7%
Lys[K] 13 7.1%
Met[M] 5 2.7%
Phe[F] 6 3.3%
Pro[P] 10 5.5%
Ser[S] 13 7.1%
Thr[T] 9 4.9%
Trp[W] 0 0.0%
Tyr[Y] 8 4.4%
Val[V] 10 5.5%
Total Length 183

Instability index
Instability index (II) 47.84
This classifies the protein as unstable

Atomic composition
Carbon 903
Hydrogen 1446
Nitrogen 242
Oxygen 279
Sulfur 5
Total number of atoms 2875
Formula = C903H1446N242O279S5

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 11920 M-1 cm-1

Aliphatic index
Aliphatic index 86.50

Coding Region Translation Calculations
Codon Bias 0.252
Codon Adaptation Index 0.224
Frequency of Optimal Codons 0.551
Hydropathicity of Protein -0.331
Aromaticity Score 0.077