VMA7/YGR020C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 9 7.6%
Arg[R] 6 5.1%
Asn[N] 5 4.2%
Asp[D] 9 7.6%
Cys[C] 0 0.0%
Gln[Q] 4 3.4%
Glu[E] 13 11.0%
Gly[G] 5 4.2%
His[H] 3 2.5%
Ile[I] 12 10.2%
Leu[L] 9 7.6%
Lys[K] 9 7.6%
Met[M] 1 0.8%
Phe[F] 7 5.9%
Pro[P] 5 4.2%
Ser[S] 3 2.5%
Thr[T] 11 9.3%
Trp[W] 0 0.0%
Tyr[Y] 2 1.7%
Val[V] 5 4.2%
Total Length 118

Instability index
Instability index (II) 41.58
This classifies the protein as unstable

Atomic composition
Carbon 601
Hydrogen 954
Nitrogen 160
Oxygen 188
Sulfur 1
Total number of atoms 1904
Formula = C601H954N160O188S1

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 2980 M-1 cm-1

Aliphatic index
Aliphatic index 89.32

Coding Region Translation Calculations
Codon Bias 0.386
Codon Adaptation Index 0.298
Frequency of Optimal Codons 0.650
Hydropathicity of Protein -0.475
Aromaticity Score 0.076