STF2/YGR008C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 1 1.2%
Arg[R] 4 4.8%
Asn[N] 7 8.3%
Asp[D] 6 7.1%
Cys[C] 0 0.0%
Gln[Q] 4 4.8%
Glu[E] 8 9.5%
Gly[G] 10 11.9%
His[H] 3 3.6%
Ile[I] 4 4.8%
Leu[L] 2 2.4%
Lys[K] 11 13.1%
Met[M] 1 1.2%
Phe[F] 3 3.6%
Pro[P] 5 6.0%
Ser[S] 5 6.0%
Thr[T] 4 4.8%
Trp[W] 2 2.4%
Tyr[Y] 2 2.4%
Val[V] 2 2.4%
Total Length 84

Instability index
Instability index (II) 32.04
This classifies the protein as stable

Atomic composition
Carbon 417
Hydrogen 644
Nitrogen 126
Oxygen 135
Sulfur 1
Total number of atoms 1323
Formula = C417H644N126O135S1

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 13980 M-1 cm-1

Aliphatic index
Aliphatic index 35.95

Coding Region Translation Calculations
Codon Bias 0.400
Codon Adaptation Index 0.339
Frequency of Optimal Codons 0.654
Hydropathicity of Protein -1.608
Aromaticity Score 0.083