Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
23 |
3.1% |
| Arg[R] |
30 |
4.0% |
| Asn[N] |
30 |
4.0% |
| Asp[D] |
40 |
5.4% |
| Cys[C] |
10 |
1.3% |
| Gln[Q] |
25 |
3.4% |
| Glu[E] |
60 |
8.1% |
| Gly[G] |
25 |
3.4% |
| His[H] |
15 |
2.0% |
| Ile[I] |
69 |
9.3% |
| Leu[L] |
84 |
11.3% |
| Lys[K] |
59 |
7.9% |
| Met[M] |
24 |
3.2% |
| Phe[F] |
29 |
3.9% |
| Pro[P] |
20 |
2.7% |
| Ser[S] |
79 |
10.6% |
| Thr[T] |
38 |
5.1% |
| Trp[W] |
8 |
1.1% |
| Tyr[Y] |
36 |
4.8% |
| Val[V] |
40 |
5.4% |
| Total Length |
744 |
|
| Instability index (II) |
48.52 |
| This classifies the protein as unstable |
|
| Carbon |
3878 |
| Hydrogen |
6140 |
| Nitrogen |
986 |
| Oxygen |
1153 |
| Sulfur |
34 |
| Total number of atoms |
12191 |
| Formula = C3878H6140N986O1153S34 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
98265 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
97640 M-1 cm-1 |
| assuming all Cys residues are reduced |
97640 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
98265 M-1 cm-1 |
|
|
| Codon Bias |
0.006 |
| Codon Adaptation Index |
0.125 |
| Frequency of Optimal Codons |
0.424 |
| Hydropathicity of Protein |
-0.202 |
| Aromaticity Score |
0.098 |
|
